UDP-N-Acetylgalactosamine: globoside a-3-N-Acetylgalactos- aminyltransferase
نویسندگان
چکیده
A UDP-N-acetylgalactosamine:globoside a-3-N-acetylgalactosaminyltransferase has been purified over 3500-fold in 4% yield from a Triton X-100 extract of canine spleen microsomes by affinity chromatography on globoside acid-agarose. Sodium dodecyl sulfate gel electrophoresis of the purified enzyme revealed two major bands with molecular weights of 66,000 and 56,000. Judging from the molecular weight of 120,000 estimated by Sephadex gel filtration in Triton X-100 and the above electrophoretic result, the enzyme presumably exists normally as a dimer. It required Mn 2+
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